Article,
Singlet oxygen quenching by riboflavin
Affiliations
- [1] Adam Mickiewicz University [NORA names: Poland; Europe, EU; OECD];
- [2] Charles University [NORA names: Czechia; Europe, EU; OECD];
- [3] Aarhus University [NORA names: AU Aarhus University; University; Denmark; Europe, EU; Nordic; OECD]
Abstract
Derivatives of riboflavin, specifically flavin mononucleotide, have recently been in the spotlight as the active component in protein-encased optogenetic sensitizers to produce singlet oxygen, O(aΔ). The extent to which riboflavin deactivates singlet oxygen is a key aspect of these studies. Surprisingly, there is a dearth of information on the rate constant for riboflavin-mediated singlet oxygen removal. We now report that, when riboflavin is dissolved in both aqueous and methanol solutions, the rate constant for riboflavin-mediated removal of singlet oxygen cannot be greater than ∼ 2 × 10 s M. This number is appreciably smaller than what has been reported in the literature and, as such, our results can be significant for mechanistic interpretations of photo-processes that involve flavins in oxygenated systems.