Article,
A 3D printable synthetic hydrogel as an immobilization matrix for continuous synthesis with fungal peroxygenases
Contributors
Meyer L.-E.
0000-0003-2591-0118
[1]
Vaupel S.
0000-0003-3536-5774
[2]
Meyer J.
0000-0003-2557-4425
[2]
Alcalde M.
0000-0001-6780-7616
[3]
Kara S.
0000-0001-6754-2814
(Corresponding author)
[1]
[2]
Affiliations
- [1] Aarhus University [NORA names: AU Aarhus University; University; Denmark; Europe, EU; Nordic; OECD];
- [2] Leibniz Universität Hannover [NORA names: Germany; Europe, EU; OECD];
- [3] CSIC [NORA names: Spain; Europe, EU; OECD]
Abstract
Enzyme immobilization is the key to an intensified bioprocess that allows recycling of the heterogenized enzyme and/or continuous biocatalytic production. In this communication, we present a case study for enzyme immobilization in a novel, 3D printable synthetic hydrogel and its use in continuous oxidation reactions. Immobilization resulted in an average immobilization yield of 6.1% and continuous synthesis was run for 24 hours with a space-time yield of 3.1 × 10 g L h
