open access publication

Article, 2024

Effects of copper nanoparticles on performance, muscle and bone characteristics and serum metabolites in broilers

In: Brazilian Journal of Biology, ISSN 1519-6984, Volume 84, 10.1590/1519-6984.261578

Contributors (8)

Abdullah S.S. [1] Masood S. (Corresponding author) [1] Zaneb H. [1] Rabbani I. [1] Akbar J. [2] Kuthu Z.H. [2] Masood A. [3] Vargas-Bello-Perez E. (0000-0001-7105-5752) (Corresponding author) [4]


  1. [1] University of Veterinary and Animal Sciences
  2. [NORA names: Pakistan; Asia, South]
  3. [2] Department of Botany, University of the Poonch Rawalakot
  4. [NORA names: Pakistan; Asia, South]
  5. [3] The Islamia University of Bahawalpur
  6. [NORA names: Pakistan; Asia, South]
  7. [4] University of Copenhagen
  8. [NORA names: Denmark; Europe, EU; Nordic; OECD]


Three hundred and twenty day old Hubbard broilers were randomly allocated to four treatments (8 replicates, 10 birds/pen) and were raised under standard management conditions. Birds in the first group served as control and were fed a corn based diet, while birds in the remaining three groups i.e.; A, B and C were fed with a basal diet supplemented with copper nanoparticles (CuNP) at 5, 10 and 15 mg/kg of diet respectively for 35 days. Supplementation of CuNP linearly increased (P≤0.05) body weight (BW), average daily weight gain (ADWG) and feed intake (FI) in broilers. Uric acid, glucose levels in blood and feed conversion ratio (FCR) reduced linearly (P≤0.05) with CuNP supplementation in diet. Supplementation of CuNP in the diet also linearly increased (P≤0.05) tibia weight, length, diameter, weight/length index (W/L) and Tibiotarsal index (TT index). Inclusion of CuNP in broilers diet linearly increased the measured parameters of muscle i.e.; pH, fiber diameter, fiber cross-sectional area, fascicle diameter, fascicle cross-sectional area (P≤0.05). Concentration of copper, iron, calcium and phosphorous in blood also increased line-arly (P ≤ 0.05) with CuNP supplementation. Overall, CuNP positively affected the growth performance, histological characteristics of muscles, bone strength and serum metabolites in broilers.


fascicle, fibre, glucose, robusticity index, tibia, uric acid