open access publication

Article, 2024

Variability in indigenous Pakistani tomato lines and worldwide reference collection for Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) infection

Brazilian Journal of Biology, ISSN 1519-6984, Volume 84, 10.1590/1519-6984.253605

Contributors

Hussain I. 0000-0002-7999-3871 (Corresponding author) [1] Farooq T. 0000-0001-7738-7993 [1] Khan S.A. 0000-0002-6662-4786 [1] Ali N. 0000-0001-8118-3678 [1] Waris M. 0000-0002-6107-704X Jalal A. 0000-0002-0218-5171 [2] Nielsen S.L. [3] Ali S. 0000-0003-3915-7390 (Corresponding author) [2] [4]

Affiliations

  1. [1] The University of Haripur
  2. [NORA names: Pakistan; Asia, South];
  3. [2] Institute of Biotechnology and Genetic Engineering
  4. [NORA names: Pakistan; Asia, South];
  5. [3] Aarhus University
  6. [NORA names: AU Aarhus University; University; Denmark; Europe, EU; Nordic; OECD];
  7. [4] Hazara University
  8. [NORA names: Pakistan; Asia, South]

Abstract

Local and exotic germplasm of tomato remains a major source for genetic improvement. Assessment of such lines for biotic stresses particularly viral diseases are the most important criteria for selection in Pakistan, where Tomato Yellow Leaf Curl Virus (TYLCV) and Tomato Mosaic Virus (ToMV) are the major diseases/viruses. A set of 40 accessions (including indigenous Pakistani lines and exotic germplasm from Europe, the United States, and Asia) were evaluated for their resistance/infection response to ToMV with artificial inoculation under greenhouse conditions. Infection response was quantified through disease scoring and DAS-ELISA test (for ToMV). A subset of 24 lines, was further screened for TYLCV using disease scoring and TAS-ELISA. The tested lines showed significant variability for resistance to ToMV. Only one accession (Acc-17878) was resistant to the ToMV whereas seven accessions i.e. Acc-17890, AVR-261, CLN-312, AVR-321, EUR-333, CLN-352, and CLN-362 expressed resistance to TYLCV. Correlation between phenotypic evaluation was confirmed by the ELISA results in both diseases, although both tools complemented to assess the viral infection status. In future, tomato breeding programs must consider breeding for ToMV and TYLCV resistance (using identified germplasm in our study) so as to deliver virus resistant tomato varieties.

Keywords

Artificial inoculation, Disease severity, ELISA, Indigenous and exotic lines, Lycopersicon esculentum

Funders

  • Higher Education Commission, Pakistan and Aarhus University

Data Provider: Elsevier